| Título | Physiological Doses of Hydroxytyrosol Modulate Gene Expression in Skeletal Muscle of Exercised Rats |
|---|---|
| Autores | CASUSO PÉREZ, RAFAEL, Al Fazazi, Saad , Plaza-Diaz, Julio , Ruiz-Ojeda, Francisco J. , Rueda-Robles, Ascension , Aragon-Vela, Jeronimo , Huertas, Jesus R. |
| Publicación externa | Si |
| Medio | Life-Basel |
| Alcance | Article |
| Naturaleza | Científica |
| Cuartil JCR | 2 |
| Cuartil SJR | 2 |
| Impacto JCR | 3.253 |
| Impacto SJR | 0.588 |
| Fecha de publicacion | 01/12/2021 |
| ISI | 000738027200001 |
| DOI | 10.3390/life11121393 |
| Abstract | We tested whether physiological doses of hydroxytyrosol (HT) may alter the mRNA transcription of key metabolic genes in exercised skeletal muscle. Two groups of exercise-trained Wistar rats, HTlow and HTmid, were supplemented with 0.31 and 4.61 mg/kg/d of HT, respectively, for 10 weeks. Another two groups of rats were not supplemented with HT; one remained sedentary and the other one was exercised. After the experimental period, the soleus muscle was removed for qRT-PCR and western blot analysis. The consumption of 4.61 mg/kg/d of HT during exercise increased the mRNA expression of important metabolic proteins. Specifically, 4.61 mg/kg/d of HT may upregulate long-chain fatty acid oxidation, lactate, and glucose oxidation as well as mitochondrial Krebs cycle in trained skeletal muscle. However, a 4.61 mg/kg/d of HT may alter protein translation, as in spite of the increment showed by CD36 and GLUT4 at the mRNA level this was not translated to higher protein content. |
| Palabras clave | polyphenols; mitochondria; glycolysis; gene expression |
| Miembros de la Universidad Loyola |