| Title |
Physiological Doses of Hydroxytyrosol Modulate Gene Expression in Skeletal Muscle of Exercised Rats |
| Authors |
CASUSO PÉREZ, RAFAEL, Al Fazazi, Saad , Plaza-Diaz, Julio , Ruiz-Ojeda, Francisco J. , Rueda-Robles, Ascension , Aragon-Vela, Jeronimo , Huertas, Jesus R. |
| External publication |
Si |
| Means |
LIFE-BASEL |
| Scope |
Article |
| Nature |
Científica |
| JCR Quartile |
2 |
| SJR Quartile |
2 |
| JCR Impact |
3.253 |
| SJR Impact |
0.588 |
| Publication date |
01/12/2021 |
| ISI |
000738027200001 |
| DOI |
10.3390/life11121393 |
| Abstract |
We tested whether physiological doses of hydroxytyrosol (HT) may alter the mRNA transcription of key metabolic genes in exercised skeletal muscle. Two groups of exercise-trained Wistar rats, HTlow and HTmid, were supplemented with 0.31 and 4.61 mg/kg/d of HT, respectively, for 10 weeks. Another two groups of rats were not supplemented with HT; one remained sedentary and the other one was exercised. After the experimental period, the soleus muscle was removed for qRT-PCR and western blot analysis. The consumption of 4.61 mg/kg/d of HT during exercise increased the mRNA expression of important metabolic proteins. Specifically, 4.61 mg/kg/d of HT may upregulate long-chain fatty acid oxidation, lactate, and glucose oxidation as well as mitochondrial Krebs cycle in trained skeletal muscle. However, a 4.61 mg/kg/d of HT may alter protein translation, as in spite of the increment showed by CD36 and GLUT4 at the mRNA level this was not translated to higher protein content. |
| Keywords |
polyphenols; mitochondria; glycolysis; gene expression |
| Universidad Loyola members |
|
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