| Title | Dual RMCE for efficient re-engineering of mouse mutant alleles |
|---|---|
| Authors | Osterwalder, Marco , Galli, Antonella , Rosen, Barry , Skarnes, William C. , Zeller, Rolf , LÓPEZ-RÍOS MORENO, JAVIER |
| External publication | Si |
| Means | Nat. Methods |
| Scope | Article |
| Nature | Científica |
| JCR Quartile | 1 |
| SJR Quartile | 1 |
| JCR Impact | 20.721 |
| SJR Impact | 8.798 |
| Publication date | 01/11/2010 |
| ISI | 000283586600008 |
| DOI | 10.1038/nmeth.1521 |
| Abstract | We have developed dual recombinase-mediated cassette exchange (dRMCE) to efficiently re-engineer the thousands of available conditional alleles in mouse embryonic stem cells. dRMCE takes advantage of the wild-type loxP and FRT sites present in these conditional alleles and in many gene-trap lines. dRMCE is a scalable, flexible tool to introduce tags, reporters and mutant coding regions into an endogenous locus of interest in an easy and highly efficient manner. |
| Universidad Loyola members |