| Autores |
Rueda, Alejandro Diaz , Salvador-Martinez, Irepan , Sospedra-Arrufat, Ismael , Alcaina-Caro, Ana , Fernandez-Minan, Ana , Burgos-Ruiz, Ana M. , Cases, Ildefonso , Mohedano, Alberto , Tena, Juan J. , Heyn, Holger , LÓPEZ-RÍOS MORENO, JAVIER, Nusspaumer, Gretel |
| Abstract |
The cellular complexity of the endochondral bone underlies its essential and pleiotropic roles during organismal life. While the adult bone has received significant attention, we still lack a deep understanding of the perinatal bone cellulome. Here, we have profiled the full composition of the murine endochondral bone at the single-cell level during the transition from fetal to newborn life and in comparison with the adult tissue, with particular emphasis on the mesenchymal compartment. The perinatal bone contains different fibroblastic clusters with blastema-like characteristics in organizing and supporting skeletogenesis, angiogenesis and hematopoiesis. Our data also suggest dynamic inter- and intra-compartment interactions, as well as a bone marrow milieu that seems prone to anti-inflammation, which we hypothesize is necessary to ensure the proper program of lymphopoiesis and the establishment of central and peripheral tolerance in early life. Our study provides an integrative roadmap for the future design of genetic and cellular functional assays to validate cellular interactions and lineage relationships within the perinatal bone.image\n Here, we used scRNA-seq to profile the complexity and molecular signatures of mouse endochondral bone cell populations before and after birth, and in comparison with the adult bone. Our integrative analysis included all mesenchymal, hematopoietic and endothelial compartments, revealing an immunomodulatory milieu within the perinatal bone, which contains several fibroblastic cell populations with multiple predicted dynamic inter- and intra-compartment interactions. This study provides a holistic framework for designing genetic tools and interpreting past data, highlighting the importance of ontogenic studies.aimage |
