| Título |
Profiling LC-DAD-ESI-TOF MS Method for the Determination of Phenolic Metabolites from Avocado (Persea americana) |
| Autores |
HURTADO FERNÁNDEZ, ELENA, Carrasco-Pancorbo, Alegria , Fernandez-Gutierrez, Alberto |
| Publicación externa |
Si |
| Medio |
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY |
| Alcance |
Article |
| Naturaleza |
Científica |
| Cuartil JCR |
1 |
| Cuartil SJR |
1 |
| Impacto JCR |
2.823 |
| Impacto SJR |
1.389 |
| Fecha de publicacion |
23/03/2011 |
| ISI |
000288401800018 |
| DOI |
10.1021/jf104276a |
| Abstract |
A powerful HPLC-DAD-ESI-TOF MS method was established for the efficient identification of the chemical constituents in the methanolic extracts of avocado (Persea americana). Separation and detection conditions were optimized by using a standard mix containing 39 compounds belonging to phenolic acids and different categories of flavonoids, analytes that could be potentially present in the avocado extracts. Optimum LC separation was achieved on a Zorbax Eclipse Plus C18 analytical column (4.6 x 150 mm, 1.8 mu m particle size) by gradient elution with water + acetic acid (0.5%) and acetonitrile as mobile phases, at a flow rate of 1.6 mL/min. The detection was carried out by ultraviolet-visible absorption and ESI-TOF MS. The developed method was applied to the study of 3 different varieties of avocado, and 17 compounds were unequivocally identified with standards. Moreover, around 25 analytes were tentatively identified by taking into account the accuracy and isotopic information provided by TOF MS. |
| Palabras clave |
Liquid chromatography; time-of-flight-mass spectrometry; phenolic compounds; avocado; Persea americana; secondary metabolites |
| Miembros de la Universidad Loyola |
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