| Título |
High-intensity high-volume swimming induces more robust signaling through PGC-1a and AMPK activation than sprint interval swimming in m. triceps brachii |
| Autores |
CASUSO PÉREZ, RAFAEL, Plaza-Diaz, Julio , Ruiz-Ojeda, Francisco J. , Aragon-Vela, Jeronimo , Robles-Sanchez, Candido , Nordsborg, Nikolai B. , Hebberecht, Marina , Salmeron, Luis M. , Huertas, Jesus R. |
| Publicación externa |
Si |
| Medio |
PLoS ONE |
| Alcance |
Article |
| Naturaleza |
Científica |
| Cuartil JCR |
1 |
| Cuartil SJR |
1 |
| Impacto JCR |
2.766 |
| Impacto SJR |
1.164 |
| Fecha de publicacion |
03/10/2017 |
| ISI |
000412131900020 |
| DOI |
10.1371/journal.pone.0185494 |
| Abstract |
We aimed to test whether high-intensity high-volume training (HIHVT) swimming would induce more robust signaling than sprint interval training (SIT) swimming within the m. triceps brachii due to lower metabolic and oxidation. Nine well-trained swimmers performed the two training procedures on separate randomized days. Muscle biopsies from m. triceps brachii and blood samples were collected at three different time points: a) before the intervention (pre), b) immediately after the swimming procedures (post) and c) after 3 h of rest (3 h). Hydroperoxides, creatine kinase (CK), and lactate dehydrogenase (LDH) were quantified from blood samples, and peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1 alpha) and the AMPK (pTHR172)/AMPK ratio were quantified by Western blot analysis. PGC-1 alpha, sirtuin 3 (SIRT3), superoxide-dismutase 2 (SOD2), and vascular endothelial growth factor (VEGF) mRNA levels were also quantified. SIT induced a higher release of LDH (p < 0.01 at all time points) and CK (p < 0.01 at post) than HIHVT, but neither SIT nor HIHVT altered systemic hydroperoxides. Additionally, neither SIRT3 nor SOD2 mRNA levels increased, while PGC-1a transcription increased at 3 h after SIT (p < 0.01) and after HIHVT (p < 0.001). However, PGC-1a protein was higher after HIHVT than after SIT (p < 0.05). Moreover, the AMPK(pTHR172)/AMPK ratio increased at post after SIT (p < 0.05), whereas this effect was delayed after HIHVT as it increased after 3 h (p < 0.05). In addition, VEGF transcription was higher in response to HIHVT (p < 0.05). In conclusion, SIT induces higher muscular stress than HIHVT without increasing systemic oxidation. In addition, HIHVT may induce more robust oxidative adaptations through PGC-1 alpha and AMPK. |
| Miembros de la Universidad Loyola |
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