| Title |
Na+-dependent D-mannose transport at the apical membrane of rat small intestine and kidney cortex |
| Authors |
DE LA HORRA PADILLA, CARMEN, Cano, M , Peral, MJ , García-Delgado, M , Durán, JM , Calonge, ML , Ilundáin, AA |
| External publication |
Si |
| Means |
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES |
| Scope |
Article |
| Nature |
Científica |
| JCR Quartile |
2 |
| SJR Quartile |
1 |
| JCR Impact |
3.243 |
| SJR Impact |
1.515 |
| Publication date |
06/06/2001 |
| ISI |
000169415700008 |
| DOI |
10.1016/S0005-2736(01)00322-4 |
| Abstract |
The presence of a Na+/D-mannose cotransport activity in brush-border membrane vesicles (BBMV), isolated from either rat small intestine or rat kidney cortex, is examined. In the presence of an electrochemical Na+ gradient, but not in its absence, D-mannose was transiently accumulated by the BBMV. D-Mannose uptake into the BBMV was energized by both the electrical membrane potential and the Na+ chemical gradient. D-Mannose transport vs, external D-mannose concentration can be described by an equation that represents a superposition of a saturable component and another component that cannot be saturated up to 50 muM D-mannose. D-Mannose uptake was inhibited by D-mannose much greater thanD-glucose > phlorizin, whereas for alpha -methyl glucopyranoside the order was D-glucose = phlorizin much greater than D-mannose. The initial rate of D-mannose uptake increased as the extravesicular Na+ concentration increased, with a Hill coefficient of 1, suggesting that the Na+:D-mannose cotransport stoichiometry is 1:1. It is concluded that both rat intestinal and renal apical membrane have a concentrative, saturable, electrogenic and Na+-dependent D-mannose transport mechanism, which is different from SGLT1. (C) 2001 Elsevier Science B.V. All rights reserved. |
| Keywords |
intestine; kidney; brush-border membrane vesicle; sodium/D-mannose |
| Universidad Loyola members |
|
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