| Title | iSuRe-Cre is a genetic tool to reliably induce and report Cre-dependent genetic modifications |
|---|---|
| Authors | FERNÁNDEZ CHACÓN, MACARENA, Casquero-Garcia, Veronica , Luo, Wen , Francesca Lunella, Federica , Ferreira Rocha, Susana , Del Olmo-Cabrera, Sergio , Benedito, Rui |
| External publication | Si |
| Means | Nat. Commun. |
| Scope | Article |
| Nature | Científica |
| JCR Quartile | 1 |
| SJR Quartile | 1 |
| JCR Impact | 12.121 |
| SJR Impact | 5.569 |
| Publication date | 22/05/2019 |
| ISI | 000468603500001 |
| DOI | 10.1038/s41467-019-10239-4 |
| Abstract | Most biomedical research aimed at understanding gene function uses the Cre-Lox system, which consists of the Cre recombinase-dependent deletion of genes containing LoxP sites. This system enables conditional genetic modifications because the expression and activity of the recombinase Cre/CreERT2 can be regulated in space by tissue-specific promoters and in time by the ligand tamoxifen. Since the precise Cre-Lox recombination event is invisible, methods were developed to report Cre activity and are widely used. However, numerous studies have shown that expression of a given Cre activity reporter cannot be assumed to indicate deletion of other LoxP-flanked genes of interest. Here, we report the generation of an inducible dual reporter-Cre mouse allele, iSuRe-Cre. By significantly increasing Cre activity in reporter-expressing cells, iSuRe-Cre provides certainty that these cells have completely recombined floxed alleles. This genetic tool increases the ease, efficiency, and reliability of conditional mutagenesis and gene function analysis. |
| Universidad Loyola members |