| Title |
Human muscular mitochondrial fusion in athletes during exercise |
| Authors |
Huertas, Jesus R. , Javier Ruiz-Ojeda, Francisco , Plaza-Diaz, Julio , Nordsborg, Nikolai B. , Martin-Albo, Jesus , Rueda-Robles, Ascension , CASUSO PÉREZ, RAFAEL |
| External publication |
Si |
| Means |
FASEB JOURNAL |
| Scope |
Article |
| Nature |
Científica |
| JCR Quartile |
1 |
| SJR Quartile |
1 |
| JCR Impact |
4.966 |
| SJR Impact |
1.866 |
| Publication date |
01/11/2019 |
| ISI |
000507461600038 |
| DOI |
10.1096/fj.201900365RR |
| Abstract |
The main objective of this work was to investigate whether mitochondrial fusion occurs in the skeletal muscle of well-trained athletes in response to high-intensity exercise. Well-trained swimmers (n = 9) performed a duration-matched sprint interval training (SIT) and high-intensity high-volume training (HIHVT) session on separate days. Muscle samples from triceps brachii were taken before, immediately after, and 3 h after the training sessions. Transmission electron microscopy (TEM) was applied to assess mitochondrial morphology. Moreover, expression of genes coding for regulators of mitochondrial fusion and fission were assessed by real-time quantitative PCR. In addition, mitofusin (MFN)2 and optic atrophy 1 (OPA1) were quantified by Western blot analysis. TEM analyses showed that mitochondrial morphology remained altered for 3 h after HIHVT, whereas SIT-induced changes were only evident immediately after exercise. Only SIT increased MFN1 and MFN2 mRNA expression, whereas SIT and HIHVT both increased MFN2 protein content 3 h after exercise. Notably, only HIHVT increased OPA1 protein content. Mitochondrial morphologic changes that suggest fusion occurs in well-adapted athletes during exercise. However, HIHVT appears as a more robust inducer of mitochondrial fusion events than SIT. Indeed, SIT induces a rapid and transient change in mitochondrial morphology. |
| Keywords |
physical activity; mitochondrial fission; skeletal muscle; mitofusin 2 |
| Universidad Loyola members |
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