| Title |
Geographical variation in serological responses to recombinant Pneumocystis jirovecii major surface glycoprotein antigens |
| Authors |
Daly, K. , Koch, J. , Respaldiza, N. , DE LA HORRA PADILLA, CARMEN, Montes-Cano, M. A. , Medrano, F. J. , Varela, J. M. , Calderon, E. J. , Walzer, P. D. |
| External publication |
Si |
| Means |
CLINICAL MICROBIOLOGY AND INFECTION |
| Scope |
Article |
| Nature |
Científica |
| JCR Quartile |
1 |
| SJR Quartile |
1 |
| JCR Impact |
4.014 |
| SJR Impact |
1.815 |
| Publication date |
01/10/2009 |
| ISI |
000270958300009 |
| DOI |
10.1111/j.1469-0691.2009.02716.x |
| Abstract |
P>The use of recombinant fragments of the major surface glycoprotein (Msg) of Pneumocystis jirovecii has proven useful for studying serological immune responses of blood donors and human immunodeficiency virus (HIV)-positive (HIV+) patients. Here, we have used ELISA to measure antibody titres to Msg fragments (MsgA, MsgB, MsgC1, MsgC3, MsgC8 and MsgC9) in sera isolated in the USA (n=200) and Spain (n=326), to determine whether geographical location affects serological responses to these antigens. Blood donors from Seville exhibited a significantly greater antibody titre to MsgC8, and significantly lower responses to MsgC3 and MsgC9, than did Cincinnati (USA) donors. Spanish blood donors (n=162) also exhibited elevated responses to MsgC1, MsgC8 and MsgC9 as compared with Spanish HIV+ (n=164) patients. HIV+ patients who had Pneumocystis pneumonia (PcP+) exhibited a higher response to MsgC8 than did HIV+ PcP- patients. These data show that geographical location plays a role in responsiveness to Msg fragments. Additionally, these fragments have utility in differentiating HIV+ PcP and HIV+ PcP+ among patient populations. |
| Keywords |
major surface glycoprotein; Pneumocystis; variation |
| Universidad Loyola members |
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