| Title |
Exploratory analysis of avocado extracts by GC-MS: new insights into the avocado fruit ripening process |
| Authors |
HURTADO FERNÁNDEZ, ELENA, Bajou, A. , Morales, J. C. , Fernandez-Gutierrez, A. , Carrasco-Pancorbo, A. |
| External publication |
Si |
| Means |
Analytical Methods |
| Scope |
Article |
| Nature |
Científica |
| JCR Quartile |
2 |
| SJR Quartile |
1 |
| JCR Impact |
1.915 |
| SJR Impact |
0.593 |
| Publication date |
01/01/2015 |
| ISI |
000360114100039 |
| DOI |
10.1039/c5ay00767d |
| Abstract |
Herewith the usefulness of a gas chromatography-electron ionization-quadrupole mass spectrometry (GC-EI-Q MS) methodology has been evaluated for the identification of potential ripening or variety markers of the avocado fruit. A selection of 13 avocado varieties at two diverse ripening degrees was made and their derivatized extracts were analyzed by GC-MS. Even though the method was able to identify about 60 metabolites within a single run, the approach used was more exploratory, and data processing and analysis steps were carried out, implying filtering, normalization, principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA). 17 compounds were selected as the most influential to be considered in PCA and clustering analysis by the k-means method. To corroborate further on the metabolic changes associated with ripening, a two class PLS-DA model was built, identifying abscisic acid, linoleic acid, aspartic acid and mannoheptulose as the compounds with the highest influence in the model. |
| Universidad Loyola members |
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