| Title |
Effect of dehydration on apical Na+-H+ exchange activity and Na+-dependent sugar transport in brush-border membrane vesicles isolated from chick intestine |
| Authors |
DE LA HORRA PADILLA, CARMEN, Calonge, ML , Ilundáin, AA |
| External publication |
Si |
| Means |
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY |
| Scope |
Article |
| Nature |
Científica |
| JCR Quartile |
1 |
| JCR Impact |
2.529 |
| Publication date |
01/06/1998 |
| ISI |
000073618700015 |
| DOI |
10.1007/s004240050611 |
| Abstract |
The current work examines the effect of 4 days of water deprivation on Na+-H+ exchange and Na+-sugar cotransport systems in brush-border membrane vesicles isolated from either the jejunum, ileum or the colon of the chick. Apical Na+-H+ exchange activity was evaluated by measuring the pH-gradient-dependent Na+ uptake. The contribution of the Na+-H+ exchangers NHE2 and NHE3 to total Na+-H+ exchange activity was evaluated from their sensitivity to the amiloride-related drug HOE694. Dehydration increased plasma aldosterone levels from 12 to 70 pg/ml and also the activities of both Na+-H+ exchange and Na+-dependent sugar transport in the, three intestinal regions tested. Na+-independent sugar transport was not modified by 4 days of water deprivation. In the ileum and colon the increase in Na+-H+ exchange activity was due to an increase in NHE2 activity, whereas in the jejunum it was due to an increase in both NHE2 and NHE3. Since we have previously reported that chronic Na+ depletion increases plasma aldosterone levels and NHE2 activity in ileum and colon, decreased small and large intestine Na+-sugar cotransport activity and had no effect on jejunal apical Na+-H+ exchange activity, it can be concluded that: (1) aldosterone does not regulate intestinal Na+-dependent sugar transport, and (2) the regulation of jejunal Na+-H+ exchange activity differs from that of either the ileum or the colon. |
| Keywords |
intestine; dehydration; Na+-sugar cotransport; Na+-H+ exchange activity; BBMV |
| Universidad Loyola members |
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