| Title | A SENSITIVE ENZYME-IMMUNOASSAY FOR ANGIOTENSIN-II IN SERUM |
|---|---|
| Authors | MARTÍNEZ LÓPEZ, JOSÉ ANTONIO, REDONDO, M , TELLEZ, T , MORELL, M |
| External publication | Si |
| Means | J. Pharm. Biomed. Anal. |
| Scope | Article |
| Nature | Científica |
| Publication date | 01/11/1994 |
| ISI | A1994PT24100008 |
| DOI | 10.1016/0731-7085(94)00082-4 |
| Abstract | A sensitive and specific enzyme immunoassay for measuring angiotensin II (AII) has been developed as a convenient alternative to a radioimmunoassay. An antiserum to AII was prepared using AII conjugated by carbodi-imide to rabbit serum albumin, and coated on to microwell plates. The labelled antigen was prepared from AII and horseradish peroxidase using the periodate method. This enzyme immunoassay was a simple two-step procedure: 0.1 ml of AII extracted plasma was incubated for 1 h at 37 degrees C; and 1 ml of labelled AII was incubated for 1 h at 37 degrees C. Bound horseradish peroxidase activity was then determined using o-phenylenediamine as chromogen by measuring the absorbance at 492 nm. The lower detection limit of the assay was 3.5 pmol l(-1). Between- and within-assay RSD values were 8.8-18.3% and 6.9-17%, respectively, for concentrations of 10-40 pmol l(-1). The accuracy of the assay, determined by recovery and linearity experiments, was 89-106% for recovery and 91-126% for parallelism. The results obtained by the present ELISA method were well correlated with those obtained by an established radioimmunoassay (n = 10, r = 0.96, intercept = 0.9 and slope = 1.02). This assay is easy to perform, rapid and does not require radioisotopes; thus it could be widely applied in clinical laboratories. |
| Keywords | ENZYME IMMUNOASSAY; ANGIOTENSIN II |
| Universidad Loyola members |