| Título | Hydroxytyrosol influences exercise-induced mitochondrial respiratory complex assembly into supercomplexes in rats |
|---|---|
| Autores | CASUSO PÉREZ, RAFAEL, Al-Fazazi, Saad , Hidalgo-Gutierrez, Agustin , Carlos Lopez, Luis , Plaza-Diaz, Julio , Rueda-Robles, Ascension , Huertas, Jesus R. |
| Publicación externa | Si |
| Medio | Free Radic. Biol. Med. |
| Alcance | Article |
| Naturaleza | Científica |
| Cuartil JCR | 1 |
| Cuartil SJR | 1 |
| Impacto JCR | 6.17 |
| Impacto SJR | 1.841 |
| Fecha de publicacion | 01/04/2019 |
| ISI | 000471822700027 |
| DOI | 10.1016/j.freeradbiomed.2019.01.027 |
| Abstract | Hydroxytyrosol (HT) has been demonstrated to improve mitochondrial function, both in sedentary and in exercised animals. Herein, we assessed the effects of two different doses of HT on exercise-induced mitochondrial respiratory complex (C) assembly into supercomplexes (SCs) and the relation of the potential results to OPA1 levels and oxidative stress. Wistar rats were allocated into six groups: sedentary (SED), sedentary consuming 20 mg/kg/d of HT (SED-20), sedentary consuming 300 mg/kg/d of HT (SED-300); exercised (EXE), exercised consuming 20 mg/kg/d of HT (EXE-20) and exercised consuming 300 mg/kg/d of HT (EXE-300). Animals were exercised and/or supplemented for 10 weeks, and assembly of SCs, mitochondrial oxidative status and expression of OPA1 were quantified in the gastrocnemius muscle. Both EXE and EXE-20 animals exhibited increased assembly of CI into SCs, but this effect was absent in EXE-300 animals. Levels of CIII2 assembled into SCs were only increased in EXE-20 animals. Notably EXE-300 animals showed a decreased relative expression of s-OPA1 isoforms. Therefore, HT exerted dose-dependent effects on SC assembly in exercised animals. Although the mechanisms leading to SCs assembly in response to exercise and HT are unclear, it seems that a high HT dose can prevent SCs assembly during exercise by decreasing the expression of the s-OPA1 isoforms. |
| Palabras clave | Exercise; Mitochondria; Oxidative phosphorylation; Polyphenols; Skeletal muscle |
| Miembros de la Universidad Loyola |